Journal: Journal of Biological Chemistry

Article Title: Disrupted glucose homeostasis and skeletal-muscle-specific glucose uptake in an exocyst knockout mouse model

doi: 10.1016/j.jbc.2021.100482

Figure Lengend Snippet: Figure 1. Conditional knockouts of Exoc5 in mouse skeletal muscle were generated. A, fluorescent microscopy evaluating tissue-specific Cre expression using a mouse line with a tdTomato (To) reporter allele. The tissue sections are from female mice. B, recombination PCRs from genomic DNA of various tissues of tamoxifen-treated male mice to detect deletion of exons 7 to 10. C, real-time quantitative PCR measuring skeletal-muscle-specific EXOC5 mRNA expression (normalized to RPLP0) in Exoc5-SMKO and CTRL mice. D, representative image of a Western blot analysis (upper panel) used for the quantification (lower panel) of EXOC5 protein levels (normalized to Vinculin) in the soleus of Exoc5-SMKO and CTRL mice. Error bars = standard deviation, *p ≤0.05, **p ≤0.01.

Article Snippet: Chem. (2021) 296 100482 After permeabilization and blocking, sections were incubated with primary antibodies (anti-insulin (Cat #66198-1-Ig, Proteintech Group, Inc); anti-glucagon (Cat # 15954-1- AP,Proteintech Group, Inc)) overnight at 4 C and incubated with appropriate fluorescent secondary antibodies (DyLight 488 Anti-Rabbit IgG and DyLight 594 Anti-Mouse, Vector Laboratories) for detection.

Techniques: Generated, Microscopy, Expressing, Real-time Polymerase Chain Reaction, Western Blot, Standard Deviation

Journal: Materials Today Bio

Article Title: Porcine pericardial decellularized matrix bilayer patch containing adipose stem cell-derived exosomes for the treatment of diabetic wounds

doi: 10.1016/j.mtbio.2024.101398

Figure Lengend Snippet: Fig. 10. The exosome-loaded bilayer decellularized pericardial patches promote the healing of diabetic refractory wounds by enhancing the activation of M2 macrophages in the wound and activating the cAMP signaling pathway. (A) On day 7, dual immunofluorescence staining for F4/80 (M0 marker), iNOS (M1 marker), and CD206 (M2 marker) was performed to assess the numbers of activated M1 and M2 macrophages in the wound and surrounding skin tissue, scale bar = 50 μm. (B) On day 7, RT-qPCR was used to detect the expression levels of M1 (cd86, il-6) and M2 (cd163, arg-1) macrophage-related genes in the wound tissue of each group. (C) On day 7, Western Blotting experiments were conducted to measure the expression levels of key proteins in the cAMP signaling pathway (Atp1a2, Calm4, and Cngb1) in the wound tissue of each group, with quantitative analysis of protein density, n = 3 (D). *, p < 0.05, **, p < 0.01, and ***, p < 0.001, compared with the PBS group.

Article Snippet: The primary antibodies used were as follows: Ki67 (13180-T48, Sino Biological Inc, China), 1:500; VEGF (ab32152, Abcam, USA), 1:2000; Atp1a2 (ab166888, Abcam, USA), 1:2000; Calm4 (CSB-PA004454GA01HU, Cusabio), 1:1000; Gngb1 (PA5-101543, Invitrogen), 1:1000; GAPDH (#2118S, CST), 1:1000.

Techniques: Activation Assay, Immunofluorescence, Staining, Marker, Quantitative RT-PCR, Expressing, Western Blot